QUESTION ON THE USE OF THE MICROSCOPE

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1. How do you put the coverslip on a slide without getting bubbles trapped under it?

2. Why do we put the slide under the spring clips on the stage of the microscope?

3. How do you adjust the amount of light coming into the microscope?

4. Using high power, would you need more or less light than when you use low power?

5. What is the difference between illumination by reflected light and by transmitted light?

6. How would you use a mirror instead of a lamp under the microscope?

7. When you are focusing on an object in which order should the three objective lenses be used?

8. When focusing on an object, why do we always start with the objective lens near the specimen?

9. How should you clean a microscope lens?

10. Calculate the total magnification of the following lenses

(a) using a X10 eye-piece with a X40 objective lens,

(b) using a X15 eye-piece with a X63 objective lens,

(c) using a X15 eye-piece with a X4 objective lens,

(d) which is more powerful: a X5 eye-piece and a X20 objective or a X7 eye-piece and a X40 objective?

11. How should a microscope be put away?

• Observing in Biology Homepage
• Observing very small specimens
• Calculating the magnification on a compound microscope
• Making a slide with a coverslip
• Looking at the slide using the microscope
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